Proteomics Related Products

Peptide Fraction Tip GL-Tip SCX, GL-Tip SDB-SCX

GL-Tip SCX is packed with strong cation polymer (SCX) and GL-Tip SDB-SCX are packed with styrene divinylbenzene polymer (SDB) and strong cation polymer (SCX). GL-Tip SDB-SCX is packed in a two layer format consisting an SDB and SCX media. Undesalted peptide samples can be used in GL-Tip SDB-SCX as the first SDB layer can desalt the sample.
The newly developed TFA gradient elution method (patent applied) identifies more peptides without decreasing operation efficiency.

Specifications

Note) Sample loading amount is an example of using AngiotensinII. Maximum sample loading amount is depending on the matrix.

Comparison of Traditional Gradient Elution VS TFA Gradient Elution

A gradient elution using cation SCX media is commonly used in shotgun proteomics to fractionate peptide samples from complex samples such as cell or tissue extracts. The biggest challenge arises when identifying the same peptide from one fractionated peptide sample to another, which results in lowering efficiency. The newly developed TFA gradient elution method (patent applied) identifies more peptides without decreasing operation efficiency.