This is an application data of analyzing L-ascorbic acid and Dehydroascorbic acid, which are known to have a Vitamin C activity and Isoascorbic acid by HPLC using PDA.
Dehydroascorbic acid is a Vitamin C compound like Ascorbic acid. Dehydroascorbic acid (DHAsA) is an oxidized form of Ascorbic acid (AsA). AsA can be detected by an UV Detector, but DHAsA can not. Therefore, it is necessary to convert the structure of the compound to make it detected by an UV Detector analyzing the total amount of Vitamin C. Also, there is an isomer of AsA known as Isoascorbic acid (ErA), which is a food additive.
This application was conducted based on the Japanese Food Sanitation Inspection Guideline.