Senna contain total sennosides (sennoside A and sennoside B), making up more than 1.0 % of the dry weight of crude drugs. The Japanese Pharmacopoeia Law, 15th Edition, requires an HPLC method for the assay and the system conformity items listed below are defined.
In this study, Senna was analyzed using a HPLC system with an Inertsil ODS-4 column using the following conditions and conformed to the system suitability test defined below.