Catecholamines play an important role as neuro-transmitter and adrenal gland hormones in the human body. Concentration of three major catecholamines (norepinephrine, epinephrine, and dopamine) in urine are significant in diagnosis and treartment of several diseases.
In this note, a determination method for urinary catecholamines using HPLC-ECD (Electrochemical detector) and MonoSpin PBA, which is a spin column for sample pretreatment, is described. The advantage of this method is not only its simplicity but also high sensitivity and selectivity. Furthermore, this method is quite easy-to-use because cleaning of conductive diamond electrode used as the working electrode can be performed routinely without removing it from the flow cell. Manual polishing of the working electrode is not necessary at all.