Capillary HPLC Columns

Physical Properties of MonoCap Series

Structure of Monolithic Silica

GL Sciences’ MonoCap series, created synthetically via sol-gel method, and an octadecyl silane chemically bonded, has a very uniform three dimensional structure that shows excellent reproducibility from batch-to-batch. The solid structure of GL Sciences’ monolithic silica eliminates the need for frits or filters at the ends of the column, thereby reducing dead volume that might otherwise lead to band broadening or sample recovery. The high porosity of our monolithic silica allows high flow rates to be used without loss of resolution or creation of high operating pressure. An optimized balance of throughpores and mesopores provides the critically important combination of efficiency, separation speed, large volume sample-loading, and small volume sample-recovery.
MonoCap High Resolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The High Resolution Ultra type deliver over 300,000 plates. The Fast-flow type is compatible with high flow rate analysis due to its’ low flow resistance. In addition, the equilibration time can be minimized further by setting the flow rate high. MonoCap Nano-flow is a high-density monolithic capillary column offering extremely high sensitivity in LC/MS due to the optimization of mesopore and throughpore sizes. MonoCap Trap columns have a relatively big throughpore, which are available for on-line pre-concentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection.

Specifications

Base Gel	: High Purity Monolithic Silica Gel
Porosity	: ≥ 85 %

Physical Properties of MonoCap Series

Description	Skeleton	Throughpore	Mesopore	Bonded Phase	End-capping	Maximum Operating Pressure
Description	Skeleton	Throughpore	Mesopore	Bonded Phase	End-capping	Maximum Operating Pressure
MonoCap C18 High Resolution 750	1 µm	2 µm	15 nm	Octadecyl	Yes	22 MPa (220 bar)
MonoCap C18 High Resolution 2000	1 µm	2 µm	15 nm	Octadecyl	Yes	35 MPa (350 bar)
MonoCap C18 High Resolution Ultra 2000	1 µm	2 µm	11 nm	Octadecyl	Yes	35 MPa (350 bar)
MonoCap C18 High Resolution 1000	1 µm	2 µm	15 nm	Octadecyl	Yes	35 MPa (350 bar)
MonoCap C18 High Resolution Ultra 1000	1 µm	2 µm	11 nm	Octadecyl	Yes	35 MPa (350 bar)
MonoCap HILIC-UP High Resolution 2000	1 µm	2 µm	12 nm	Ureidopropyl	None	35 MPa (350 bar)
MonoCap C18 Fast-flow	1 µm	2 µm	15 nm	Octadecyl	Yes	22 MPa (220 bar)
MonoCap C18 Nano-flow	1 µm	1 µm	11 nm	Octadecyl	Yes	22 MPa (220 bar)
MonoCap C18 WideBore	1 µm	2 µm	11 nm	Octadecyl	Yes	22 MPa (220 bar)
MonoCap C18 Trap Column	2 µm	5 µm	11 nm	Octadecyl	Yes	20 MPa (200 bar)
MonoCap Amide	1 µm	2 µm	15 nm	Carbamoyl	None	22 MPa (220 bar)