Objectives of Solid Phase Extraction (SPE)

How to Select a Sorbent

How to Select a Sorbent Depending on the Sample Matrix and Target Analyte

One of the most important elements to achieve successful of solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte.
The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.

(1) Ion Exchange Phase (SAX, SCX, etc.)
(2) Specialty Phase (PBA, etc.)
(3) Mix Phase (Certify, MPC, etc.)
(4) Polar Phase (SI, FL, etc.)
(5) Non-Polar Phase (Active Carbon, SDB, C18, etc.)

Selectivity of SPE

In order to effectively utilize the solid phase extraction method, it is important to choose a sorbent amount and size appropriate to the loading amount of the matrix and the desired product. Generally, the total retention of the cartridge type (including contaminants) is said to be up to 5 % of the solid phase loading.
In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.

Retentive Capacity of a Sorbent Compared to Sorbent Mass

※Bed volume, this is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume

Recommendation for Selecting an Ion Exchange Sorbent